Maelle Locatelli2, Chloé Hommais1, Fadil Iqbal3, Keith Bonin4, Kerry Bloom2, Jing Liu3, and Pierre-Alexandre Vidi1
1 Institut de Cancérologie de l’Ouest, Angers, France
2 Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, USA
3 Department of Physics, Purdue University, West-Lafayette, USA
4 Department of Physics, Wake Forest University, Winston-Salem,...
The loop-extrusion process has emerged as a pivotal mechanism governing chromosome organization within the nucleus. However, the impact of this process on the effective stiffness of chromosomes remains largely unexplored. In this study, we introduce a novel polymer model to investigate the force-extension behavior of chromosomes with loop-extrusion activity. Our analytical and simulation...
The bacterial DNA segregation is mainly performed with the ParABS system. It is composed of ParB, a binding protein, ParA, an ATPase and parS, a specific binding DNA sequence that ParB binds parS with high affinity. Hundreds of ParB are recruited around parS into a complex, called ParBS, which displays liquid-like properties.
Recently, it has been shown that ParB is using energy stored as...
Claudie Carron1, Carine Dominique1, Nana Kadidia Maiga 1, Mickaël Lelek2, Thomas Mangeat3, Frédéric Beckouët1, Christian Rouvière3, Isabelle Léger-Silvestre1, Sylvain Cantaloube3, Christophe Zimmer2, Anthony K. Henras1, Benjamin Albert1and Olivier Gadal1.
1: MCD (CBI), Université de Toulouse, CNRS, UPS, 31000, Toulouse, France
2: Imaging and Modeling Unit, Department of...
Uracil can exist in dsDNA by spontaneous cytosine deamination or by misincorporation of dUMP instead of dTMP during the replication process [1]. A highly specific Uracil-DNA glycosylase (UDG) enzyme recognizes and excise the flipped uracil base from the dsDNA helix; this mechanism is part of the base-excision repair (BER) pathway [2]. There remains a lack of complete understanding in the...
Coordina3ng chromosome segrega3on with cell division is a key-step of the cell cycle for any bacteria. E. coli does not seem to require an ac3ve system to segregate its chromosomes. Instead, entropy and a specific organiza3on of the chromosome into domains seem to explain the observed choreography. MatP is the DNA-binding protein responsible for organizing the ter region by preven3ng the SMC...
In bacteria, low-copy-number replicons carry self-specific partition systems to ensure their faithful segregation. Among these systems, ParABS partition systems, consisting of a Walker-type ATPase (ParA) and a DNA-binding protein (ParB) along with parS centromere sites, are the most prevalent on plasmids and the only one present on chromosomes. ParB proteins, recently shown to belong to a...
The rewiring of glucose metabolism from mitochondrial respiration to
fermentation is a hallmark of cancer. This metabolic adaptation has been exploited in
ongoing therapeutic and diagnostic strategies. Notably, treating cancer cells with the
glucose analog 2-deoxy-D-glucose (2DG) reduces their proliferation presumably by
dampening the rate of glycolysis. However, this treatment leads to...
Study of the role of the bacteriophage T4 protein, Ndd in the disorganization of the bacterial nucleoid in E. coli
Justine Groseille1, 2 (groseillejustine@gmail.com),Cristian Ilioaia4, Agnès Thierry2, Cristian Ilioaia4, Romain Koszul2, Olivier Espéli1
1 Dynamique des chromosomes - CIRB, College de de France, PARIS, France
2 UMR3525- Regulation spatiale des génomes, Institut Pasteur,...