Cinquième réunion annuelle du GDR Architecture et Dynamique du Noyau et des Génomes (ADN&G)

Poster : Applying Latent Dirichlet Allocation(LDA) to decompose single-cell chromatin conformations

Non programmé

20m

amphithéâtre (GENOPOLYS)

Orateur

Jean-Bernard Fiche (Centre de Biologie Structurale)

Description

Chromatin structure varies dramatically between cells, but the specific
motifs that underlie this organization remain poorly understood. Using
imaging-based chromatin tracing techniques, we visualized 3D chromatin
organization in mouse pancreatic tissue. We show that individual
chromatin traces can be decomposed into loop and domain chromatin
folding motifs (CFMs) using standard Latent Dirichlet Allocation (LDA)
topic-modeling methods. Polymer simulations revealed that loop extrusion
reproduces loop CFMs, while cis-regulatory interactions are primarily
responsible for domain CFMs.