5ème Rencontre scientifique des Grands Causses

Diapos des présentations

3. Chromatin structure from high resolution microscopy: scaling laws and micro-phase separation

M. Loucif Remini (L2C)

Oral

Recent advances in experimental fluorescence microscopy allow high accuracy determination (resolution of 50nm) of the 3D physical location of multiple (up to $~10^2$) tagged regions of the chromosome. We investigate publicly available microscopy data for two loci of the human genome obtained from multiplexed FISH methods for different cell lines and treatments. Inspired by polymer physics...

6. Order in the disordered - Molecular determinants of phase separation and its physiological role in plant temperature sensing

Dr Stephanie Hutin (LPCV/CNRS)

Oral

Order in the disordered - Molecular determinants of phase separation and its physiological role in plant temperature sensing

S. Hutin, JR. Kumita, P.Wigge, L. Costa, M. Tully, C. Zubieta

As sessile organisms, plants must live with constant environmental fluctuations and stress constraints. The increased average temperature and prolonged periods of extreme weather due to climate alter...

2. PRC1 nano-structures compact Polycomb-associated chromatin during Drosophila embryogenesis.

Thierry Cheutin (CNRS)

Oral

Regulation of gene expression during cell differentiation is a complex process involving several levels of chromatin organization. Transcription factors such as promoters and enhancers act on discrete elements whose accessibility can be controlled by nucleosomes. Polycomb grouped proteins (PcG) are key factor required to maintain silenced chromatin state. Although PcG proteins bind discrete...

7. The bacterial DNA segregation complexes ParBS display a twofold phase separation

Dr Jean-Charles Walter (L2C, CNRS & Montpellier Université)

Oral

The bacterial DNA segregation is prevalently performed through the ParABS system for most chromosome and low-copy number plasmids. It consists in two proteins: (i) ParB a DNA binding protein with a specific binding site at parS. It has been recently found to be a CTP-ase; (ii) ParA, an ATP-ase whose ATP hydrolysis is catalyzed by ParB and (iii) parS, a centromere-like sequence where ParB can...

8. The cell cycle as a phase separation cycle controlled by protein phosphorylation

Liliana Krasinska (IGMM CNRS)

Oral

5. The RD-HRS Method : New insights on the relationship between phase separation, DNA and RNA

Nathan Lecouvreur (IGMM)

Oral

The RD-HRS method: new insights on the relationship between phase separation, DNA and RNA

Nathan Lecouvreur1, Cosette Rebouissou1, Annick Lesne1,2, Thierry Forné1

1 Institut de Génétique Moléculaire de Montpellier, UMR5535, CNRS-Université de Montpellier, 1919 Route de Mende, 34293 Montpellier Cedex 5, FRANCE.
2 Laboratoire de Physique Théorique de la Matière Condensée, CNRS UMR 7600,...

1. Transcription regulates the spatio-temporal dynamics of genes through micro-compartmentalization

Dr hossein salari (Laboratory of Biology and Modelling of the Cell, Univ Lyon, ENS de Lyon)

Although our understanding of the involvement of heterochromatin architectural factors in
shaping nuclear organization is improving, there is still ongoing debate regarding the role of
active genes in this process. In this study, we utilize publicly-available Micro-C data from mouse
embryonic stem cells to investigate the relationship between gene transcription and 3D gene
folding. Our...

4. Unraveling the principles regulating chromosome spatial organization during differentiation

Marco Di Stefano (Institut de Génétique Humaine Montpellier)

Oral

Chromosomal structural organization contributes to fundamental processes in the cell nucleus, including DNA transcription, replication, and repair. Experimental and theoretical works unveiled that chromosome organization consists of a complex aggregate of layers: entire chromosomes occupy distinct regions of the nucleus, called territories; below, at the tens of Mega-bases (Mb) scale, active...