Molecular dynamics insights for DNA lesions in oligonucleotides and nucleosomal DNA

Non programmé
20m

Orateur

Elise Dumont (LCH, UMR 5182, ENS de Lyon)

Description

The identification of complex oxidatively-generated DNA lesions involving not only a single-nucleobase but two proximal nucleotides has recieved much attention over the last years since such lesions are formed with a very low occurrence (typically a few lesions per 10^9 nucleotides) but turn out to be highly mutagenic. The structural elucidation of such complex lesions is extremely challenging.

We rely on classical all-atom MD simulations to probe the structures of oligonucleotides featuring a given lesion (abasic sites or intrastrand cross-links). We can monitor the B-helix distortion and the outcome of the initial Watson-Crick pairing: whereas NMR or X-ray structures are not available, our simulation provide a rationale for sequence-dependent repair efficiencies. Another application of all-atom molecular dynamics aspect is the exploration of DNA-photosensitizers interactions.

I will also present our recent efforts towards all-atom simulations of nucleosomal harbouring DNA lesions.

Auteur principal

Elise Dumont (LCH, UMR 5182, ENS de Lyon)

Co-auteur

Dr Emmanuelle Bignon (ISA Univ Lyon 1)

Documents de présentation

Aucun document.