Développement méthodologique de spectrométrie de masse pour l’analyse protéomique quantitative : Evaluation de la compatibilité du couplage de séparation de protéines par gel d’électrophorèse SDS-PAGE avec une quantification par LC-SRM (by Alvaro Vaca Jacome - DSA) Room: Amphiteatre Grünewald Le dosage précis et sensible de dizaines, voire de centaines de protéines dans un mélange contenant plusieurs milliers de protéines devient une étape incontournable pour répondre à de nombreuses questions biologiques. Cette méthode passe par l’utilisation d’un appareil de type MS-MS pour analyser le mélange de peptides issu de la digestion de l’extrait protéique total. De très nombreux paramètres (relatifs à la préparation d’échantillon et à l’instrumentation de la spectrométrie de masse) sont à optimiser pour obtenir les sélectivités et les sensibilités nécessaires pour la quantification de protéines cibles dans des mélanges complexes. Dans ce contexte, nous voulons évaluer la compatibilité du couplage entre une méthode de séparation de protéines (Gel SDS-PAGE) avec une méthode de quantification par couplage chromatographie liquide/spectrométrie de masse de type triple-quadruple.

Supersymmetry in high energy particle physics (by Adam Alloul - DRS) Room: Amphiteatre Grünewald Ph.D student in high energy particle physics, my work is twofold. On the physics side, it consists in studying theoretical models and providing to the experimentalists the characteristic signatures they could look for at the Large Hadron Collider (LHC). On the other side, I develop automated tools to make this kind of investigations easier. During my talk, I will try to explain the big questions to which particle physicists want an answer and show you how this can be done.

Following animal’s finest movements (by Gaëlle Fehlmann - DEPE) Room: Amphiteatre Grünewald Gaëlle Fehlmann, C. Bodin and Y. Handrich Wild animals often raise some important issues, because of biodiversity decline or their unexpected interaction with our infrastructures or activities. Thus, behavior of free ranging animal is a key to a global comprehension of biological mechanism and conservation. The usual way to study behavior is to directly observe them, but how to follow them in their sub-aquatic activities or in their burrows? Devices directly attached to animals (Bio-logging) are today used to overpass this limitation and one way to study behavior is the use of tridimensional accelerometers. Recording acceleration in the three dimensions it is possible to get information about animal’s posture and their body acceleration and then define their time budget.

Combination of Anatomical and Functional Information for a Small Animal Preclinical Imaging System (by Harold Barquero - DRS) Room: Amphiteatre Grünewald Integrated imaging systems are increasingly used in preclinical research because of their ability to provide both morphological and functional information. Since a few years, a novel type of functional imaging modalities for small animal, called optical imaging, make possible to get a functional information while reducing mainly the acquisition time and the system cost. These modalities can provide a valuable qualitative information, but improvements are to be done regarding their quantitative aspect. I will give an overview of my work which consists in the integration of an anatomical information to improve quantification capabilities of optical imaging modalities.