Orateur
Description
I present an ongoing investigation on whether the electrostatic potential across the nuclear membrane or its fluctuations influences chromatin organization directly via condensation or indirectly through confinement and crowding, focusing on Saccharomyces cerevisiae across its growth cycle. In particular, we target key metabolic transitions such as glucose uptake, and will investigate the diauxic shift and entry into quiescence (Q phase), where major changes in nuclear architecture and metabolism occur. To test this, we are developing tools to quantify the electrical properties of the inner nuclear membrane and track their dynamics during these transitions. Using chemical and genetic perturbations of metabolic pathways, we will assess how shifts in membrane potential affect chromatin structure by high-resolution live imaging. Biophysical models will predict how electrostatic and crowding effects can shape chromatin conformation, the integration of models with experiments will allow the validations of hypothesis. This integrative approach aims to uncover how nuclear electrostatics modulate chromatin folding and cellular function during yeast growth phases.
