Orateur
Description
In bacteria, genetic context can have significant impact on gene expression but is currently not integrated in quantitative models of gene regulation despite known biophysical principles and quantitative in vitro measurements. Conceptually, the simplest genetic context consists of a single gene framed by two topological barriers, also known as the twin transcriptional-loop model, which illustrates how transcription both affects and is affected by DNA supercoiling. In vivo, DNA supercoiling is additionally modulated by topoisomerases whose modus operandi remains to be quantified.
In this talk, I will first present an experimental realisation of the twin transcriptional-loop model in Escherichia coli. I will then show how a first-principle biophysical model of DNA transcription accounts quantitatively for all the data and makes predictions on topoisomerase activity in vivo.
