Réunion annuelle du GDR ADN & Rencontre scientifique des Grands Causses

Diapos des présentations

2. Characteristic lengths in bacterial genomes

Ivan Junier (TIMC-IMAG, CNRS)

In condensed matter physics, simplified descriptions are obtained by coarse-graining the features of a system at a certain characteristic length, defined as the typical length beyond which some properties are no longer correlated. From a physics standpoint, in vitro DNA has thus a characteristic length of 300 base pairs (bp), the Kuhn length of the molecule beyond which correlations in its...

14. Conférence grand public: Le Projet Lutétium

Mathias Casiulis (LPTMC, Sorbonne Université)

Le Projet Lutétium est un projet vidéo à la rencontre de la recherche expérimentale, de la médiation scientifique, et des arts musical et graphiques. Via sa chaîne YouTube, il vise à faire découvrir par des expériences visuelles des sujets de recherche actuels et peu communs en communication grand public. Pour l'événement, une présentation du Projet accompagné de projections de vidéos...

1. Cryo - EM evidence of nucleosome conformational changes in concentrated solutions and in interphase nuclei

Amélie Leforestier (Laboratoire de Physique des Solides CNRS UMR 8502, Orsay, France)

In Eukaryotes, DNA is wound around the histone core octamer to form the basic chromatin unit, the nucleosome. Atomic resolution structures have been obtained
from crystallography and single particle cryoEM, with identical reconstituted particles. However, native nucleosomes are diverse in DNA sequence and histone content, and the conformational variability of native nucleosomes remains to be...

7. Dynamic interplay between enhancer-promoter topology and gene activity

Thomas Gregor

A long-standing question in gene regulation is how remote enhancers communicate with their target promoters, and in particular how chromatin topology dynamically relates to gene activation. Here, we combine genome editing and multi-color live imaging to simultaneously visualize physical enhancer–promoter interaction and transcription at the single cell level in Drosophila embryos. Examining...

15. Effect of epigenetics and extrusion on chromatin organization and dynamics

Surya Ghosh (Post Doc)

12. Effect of gene position in the genome and in the cell on transcription regulation

Bianca Sclavi (LBPA, UMR 8113)

9. Imaging nanoscale chromatin compaction in vivo

David LLERES (CNRS UMR5535)

How metazoan genomes are structured at the nanoscale in living cells and tissues remains largely unknown. In addition, it still remains difficult to explore chromatin in vivo, particularly at both nucleosomal array level and single cell definition. Here, we applied a quantitative FRET (Forster resonance energy transfer)-based fluorescence lifetime imaging microscopy (FLIM) approach to measure...

10. Looping and Clustering model for the organization of protein-DNA complexes on the bacterial genome

Nils-Ole Walliser (L2C, Univ Montpellier, CNRS, Montpellier, France)

The bacterial genome is organized by a variety of associated proteins inside a structure called the nucleoid. These proteins can form complexes on DNA that play a central role in various biological processes, including chromosome segregation. A prominent example is the large ParB-DNA complex, which forms an essential component of the segregation machinery in many bacteria. ChIP-Seq experiments...

11. Modélisation Multi-échelle de l'Architecture Nucléaire

Antony Lesage (LPTMC)

8. Multiscale Structuring of the E. coli Chromosome by Nucleoid-Associated and Condensin Proteins

Virginia Lioy (I2BC)

As in Eukaryotes, bacterial genomes must be accurately folded to ensure their proper function. Bacterial genetic information is generally carried on a single circular chromosome with a unique origin of replication from which two replication forks proceed bidirectionally to the opposite terminus region. Here we investigated the higher-order genome architecture of the model bacteria Escherichia...

4. Numerical model of checkpoint dependent replication origin activation in the Xenopus in vitro system

Diletta Ciardo

The initiation of DNA replication in multicellular organisms begins at thousands of genomic positions known as replication origins, which are activated at different times during the S phase in a regulated manner. Furthermore, few origins are grouped into so-called replication clusters that fire more or less synchronously. Previous studies point out that in the Xenopus in vitro system the...

5. Polycomb proteins and 3D genome folding in the epigenetic regulation of development

Giacomo Cavalli (CNRS)

The eukaryotic genome folds in 3D in a hierarchy of structures, including nucleosomes, chromatin fibers, loops, chromosomal domains (also called TADs), compartments and chromosome territories that are highly organized in order to allow for stable memory as well as for regulatory plasticity, depending on intrinsic and environmental cues. Polycomb Group (PcG) and trithorax group (trxG) proteins...

3. Predicting double-strand DNA breaks using epigenome marks or DNA at kilobase resolution

Raphael Mourad (Université Paul Sabatier)

Background

Double-strand breaks (DSBs) result from the attack of both DNA strands by multiple sources, including exposure to ionizing radiation or reactive oxygen species. DSBs can cause abnormal chromosomal rearrangements which are linked to cancer development, and hence represent an important issue. Recent techniques allow the genome-wide mapping of DSBs at high resolution, enabling the...

6. Stochasticity in drosophila chromosome organization

marcelo nollmann (CNRS)

At the kilo- to megabase pair scales, eukaryotic genomes are partitioned into self-interacting modules or topologically associated domains (TADs) that associate to form nuclear compartments. Here, we combine high-content super-resolution microscopies with state-of-the-art DNA-labeling methods to reveal the variability in the multiscale organization of the Drosophila genome. We find that...

13. Transcription et dynamique de la fibre de chromatine

Olivier Gadal (CNRS)